ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials.
ASTM E – Method Determining the Antimicrobial Activity
Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. Active view current version of standard.
Summary of Test Method: Test Package Request Form. This website stores cookies on your computer.
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This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour. No regulated reduction limits currently exist for general antimicrobial claims made using this method. This test method is only intended to determine efficacy as r2149 in 4.
Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use. The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.
These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.
The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.
Historical Version s – view previous versions of standard. Link to Active This link will always route to asym current Active version of the standard. Record and report presence of solution activity.
These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility. One-hour contact time in a buffer solution allows for metabolic stasis in the population.
Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.
This standard does not purport to address all of the safety concerns, if any, associated with its use. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors. Work Item s – proposed revisions of asstm standard.
ASTM E2149 Antimicrobial Activity
To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.
The presence of a leaching antimicrobial is determined post-test. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent wstm dependent upon direct contact of microbes with the active chemical agent.
ASTM E – Microbe Investigations (MIS)
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It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 e214 insoluble compounds will not.
Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome asmt in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
This standard does not purport to address all of the safety concerns, if any, associated with its use. No other units of measurement are included in this standard.